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Foregene EndoFree Plasmid Maxi Kit

Glass cellulose membrane efficiently and specifically binds plasmid DNA
The EndoFree Maxi Plasmid Kit adopts a unique silica membrane 
adsorption technique to efficiently and specifically bind plasmid DNA.

By combining the special Endotoxin Removal Buffer P4 and EndoFree Maxi 
Filtration Columns, endotoxin, proteins and other impurities can be 
effectively removed. The whole extraction process takes only 1 hour, 
ensuring the convenient and quick operation. The plasmid DNA extracted 
by this kit can be applied to various routine operations, including enzymatic 
digestion, PCR, sequencing, ligation, transformation as well as the transfection for various cell types.

| Description

Recommended bacteria amount for each practice: For high-copy plasmid, 
it is recommended to use 100 ml of bacteria culture media, and the yield 
from which is generally around 500-1500 μg. For low-copy plasmid, 200 ml 
bacteria culture media is recommended to generate around 200-600 μg 
plasmids.

| Features

● Fast and high-yield: 200 μg-1.5 mg plasmids can be extracted in about 1 hour, and there 
are many supercoiled plasmids.
Efficient transfection: suitable for advanced transfection experiments of most cell lines.
Widerangeofapplications:suitableforroutineexperimentssuchasrestrictiondigestion,
| Specification
PCR, sequencing, connection, transformation,etc.
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This kit can be used for various routine operations, including enzyme 
digestion, PCR, sequencing, ligation, transformation, library screening, in 

| Application

vitro translation, and transfection of some routine passaged cells.
RNase A is shipped at room temperature and stored at (2-8)° C upon receipt; 
other components are stored in a dry condition at (15-25)° C for
| Storage
12 months.

| Case

Experimental material: Escherichia coli bacteria liquid
Experimental method: Use FOREGENENE D909 kit to extract E. coli 
plasmid DNA with an initial volume of 200mL. 100μL Buffer EB was 
eluted and detected by agarose gel electrophoresis. The results are as 
above.

Cat.No. Specification
DMO-005E-D909-E 10 Preps
42