¿µÀÎÇÁ·±Æ¼¾î¢ß¿¡¼­ Western blotting ÀÇ ½Å·ÚÇÒ ¼ö ÀÖ´Â data ¸¦ ¾ò±â À§ÇÏ¿© °íǰÁúÀÇ Loading control, Secondary antibody ¿Í WESTSAVE upTMÀ» Á¦°øÇϰí ÀÖ½À´Ï´Ù. ±¹»ê Ç×ü¿Í ÇÔ²² ÇØ¿Ü Àú¸í ÇмúÁö¿¡ ³í¹®À» °ÔÀçÇÏ´Â Áñ°Å¿òÀ» ¾òÀ¸½Ã±æ ¹Ù¶ø´Ï´Ù.

Loading Control, 2nd Antibody, WESTSAVE upTM ´ëÇѹα¹ Á¦Ç°À¸·Îµµ ÃæºÐÇÕ´Ï´Ù.
  • Àú¸íÇÑ ÇмúÁö °ÔÀç (Cell, PNAS µî ´Ù¼ö)
  • °¡Àå °æÁ¦ÀûÀÎ °¡°Ý (WESTSAVE upTMÀÇ °æ¿ì G »ç ´ëºñ
       1/16 °¡°Ý)
  • °íǰÁú (Á¤È®ÇÑ Detection Á¦°ø)
  • Loading control

    Anti-GAPDH

    Name

    Clonality

    X-reactivity

    Application

    Cat No.

      Anti-GAPDH

    Poly

    Hu, Ms, Rt

    WB, IP

    LF-PA0018

      Anti-GAPDH

    Poly

    Hu, Ms, Rt

    WB, IF, IHC

    LF-PA20176

      Anti-GAPDH

    Mono

    Hu

    E, WB, IP

    LF-MA0038

    Anti- ¥á tubulin

    Name

    Clonality

    X-reactivity

    Application

    Cat No.

      Anti- ¥á tubulin

    Poly

    Hu, Ms, Rt

    WB

    LF-PA0146

      Anti- ¥á tubulin

    Mono

    Hu, Ms, Rt

    WB

    LF-MA0117

    Anti- ¥â actin

    Name

    Clonality

    X-reactivity

    Application

    Cat No.

      Anti- ¥â actin

    Poly

    Hu, Ms, Rt

    WB

    LF-PA0207

      Anti- ¥â actin

    Poly

    Hu

    WB

    LF-PA20054

      Anti- ¥â actin

    Mono

    All

    WB, IF

    LF-MA20055

      Anti- ¥â actin

    Poly

    Hu, Ms, Rt

    WB, IF, IHC-P

    LF-PA40012


    <REFERENCE>

    1. Shim JH. et. al. (2006) Proteomics. 6(1):81-93 (GAPDH)
    2. Wong JJ. et. al. (2006) PNAS. 103(28):10735-40 (GAPDH)
    3. Han J. et. al. (2009) Cell 136: 75-84 ( ¥á tubulin)
    4. Heo I. et. al. (2008) Mol Cell 32:276-284 ( ¥á tubulin)
    5. Choi I. et. al. (2008) Mol Immunol. 45(2):575-80 ( ¥á tubulin)

    Secondary antibody

    Goat anti-mouse IgG (HRP)

    Cat No.

    Specificity

    Application

    Size

     LF-SA5001

    ¥ã chain

    WB, IHC, ICC, Direct ELISA

    200 ug

     LF-SA5001A

    500 ug

    Goat anti-rabbit IgG (HRP)

    Cat No.

    Specificity

    Application

    Size

     LF-SA5002

    ¥ã chain

    WB, IHC, ICC, Direct ELISA

    200 ug

     LF-SA5002A

    500 ug

    WESTSAVE upTM

    AbFrontier WESTSAVE upTM(Western Blotting Detection Kit) is enhanced luminol-based chemiluminescent substrate for the non-radioactive detection of Horseradish Peroxidase (HRP) labeled antibodies

    WESTSAVE up : LF-QC0101

     Detection reagent A (Luminol enhance solution)

    200mL

     Detection reagent B (peroxide solution)

    1mL

    WESTSAVE up, double pack : LF-QC0102

     Detection reagent A (Luminol enhance solution) x2

    200mL x2

     Detection reagent B (peroxide solution) x2

    1mL x2

    <REFERENCE>
    1. van den Bemd GJ. et. al (2006) Mol Cell Proteomics. 5(10):1830-9
    2. Choi K. et. al. (2007) FEBS Lett. 581(24):4691-6
    3. Lee KH. et. al. (2008) Mol. Cells. 25(1):70-77

    <High sensitivity>               <Long signal duration time>


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