All kit components of this kit are stable at 2 to 8°C. Any unused reconstituted standard should be discarded or frozen at -70°C. Standard can be frozen and thawed one time only without loss of immunoreactivity.
< 1.0052 pg/ml
Sample Type :
Human serum, plasma, cell lysate, culture supernatants, buffered solution
IL-2, a soluble hormone-like mediator of the immune system, was the first interleukin molecule to be identified and characterized. Subsequently, IL-2 was discovered to be a member of a family of cytokines, which also includes IL-4, IL-7, IL-9, IL-15 and IL-21. IL-2 signals through a receptor complex consisting of IL-2 specific IL-2 receptor alpha (CD25), IL-2 receptor beta (CD122) and a common gamma chain (γc), which is shared by all members of this family of cytokine receptor. IL-2 is necessary for the growth, proliferation, and differentiation of T cells to become 'effector' T cells. IL-2 is normally produced by T cells during an immune response. Antigen binding to the T cell receptor (TCR) stimulates the secretion of IL-2, and the expression of IL-2 receptors IL-2R. The IL-2/IL-2R interaction then stimulates the growth, differentiation and survival of antigen-specific CD4+ T cells and CD8+ T cells As such, IL-2 is necessary for the development of T cell immunologic memory, which depends upon the expansion of the number and function of antigen-selected T cell clones. IL-2 is also necessary during T cell development in the thymus for the maturation of a subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against self antigens, which could result in autoimmunity. T-Regs do so by preventing the responding cells from producing IL-2. Also, because T-Reg cells constitutively express IL-2 receptors, they bind, internalize and degrade IL-2, thereby depriving neighboring effector T cells of IL-2. Thus, IL-2 is required to discriminate between self and non-self, one of the other hallmarks of the immune system.
Background reference :
1) Smith KA, Lachman LB, Oppenheim JJ, Favata MF (June 1980). "The functional relationship of the interleukins". J. Exp. Med. 151 (6): 1551–6.
2) Smith KA, Gilbride KJ, Favata MF (October 1980). "Lymphocyte activating factor promotes T-cell growth factor production by cloned murine lymphoma cells". Nature 287 (5785): 853–5.
3) Robb RJ, Smith KA (December 1981). "Heterogeneity of human T-cell growth factor(s) due to variable glycosylation". Mol. Immunol. 18 (12): 1087–94.
4) Smith KA, Favata MF, Oroszlan S (October 1983). "Production and characterization of monoclonal antibodies to human interleukin 2: strategy and tactics". J. Immunol. 131 (4): 1808–15.