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Antibodies are reagents used in ELISA, Western blotting, Immuprecipitation,
Immunohistochemistry and Immunocytochemistry for having the property of antigen
specific affinity.
AbFRONTIER's antibody production service using rabbits and mouse helps our
clients with the antigen preparation and antibody purification that along with
the production service can expand the purpose and use of antibodies for our
clients. |
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| Production service that provides polyclonal antibody using rabbits. |
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AbFRONTIER Service Features
- Immunization using 1 + 1 rabbits
- To provide (>) 50 ㎖ serum per rabbit.
- To provide with the preimmune serum and with two test serums (4th
and 6th week).
- To report three times on the result of test serum titer
measurements (ELISA Test).
- Optional Western blotting or Immunoprecipitation test available
- Period: 10 weeks
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Client Requirements
- Protein 2 ㎎ (purity : 90-95%) or peptide 2.5 ㎎ (purity : about 70%)
- Antigen information (M.W., toxicity , buffer, concentration etc.)
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Protocol
1. Preparation of experimental animals
2. Preparation of antigen
3. Preimmune bleeding & Primary injection
4. 3 times boosting & 2 times test serum bleeding, ELISA test
5. Whole blood drawing
6. Serum separation
7. Cell freezing
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| Polyclonal antibody production service using mice |
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AbFRONTIER Service Features
- Immunization using 5 BALB/c mice
- To provide 0.5-1 ㎖ serum per mouse
- To provide two reports on test serum titer measurements (ELISA
test).
- Optional Western blotting or Immunoprecipitation test available
- Period: 10 weeks
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Client Requirements
- Protein 2.5 ㎎ (purity : 90-95%) or peptide 5 ㎎ (purity
: about 70%)
- Antigen information (M.W., toxicity, buffer, concentration etc.)
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Protocol
1. Preparation of experimental animals
2. Preparation of antigen
3. Preimmune bleeding & Primary injection
4. 3 times boosting & 2 times test serum bleeding, ELISA test
5. Drawing of whole blood
6. Serum separation
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| Production service for monoclonal antibody using mice. |
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AbFRONTIER Service Features
- Immunization using 5 BALB/c mice
- Various screening methods available: ELISA test, Western
blotting, Immunoprecipitation
(When choosing Western blotting or Immunoprecipitation,
clients are required to prepare
the test samples and the certain fees will be charged.)
- When requested, culture supernatant provided for test
- Minimum two positive clones provided according to the screening
methods
requested by the client.
- Ascites production available using hybridoma cells. (for an extra
charge)
- Period: 18-26 weeks
- The period can be shorter based on the immunization methods.
(Inquire for more information)
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Client Requirements
- Protein 2.5 ㎎ (purity : 90-95%) or peptide 5 ㎎ (purity
: about 70%)
- Antigen information (M.W., toxicity, buffer, concentration etc.)
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Protocol
1. Preparation of experimental animals
2. Preparation of antigen
3. Preimmune bleeding & Primary injection
4. Immunity test
5. Fusion
6. Screening
7. Cell freezing
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| High-grade purification service using serum or ascites. |
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| AbFRONTIER Service Features |
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| Methods |
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Column chromatography (2 step) |
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Column chromatography (1 step) |
Chromatography
resin |
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1) Antigen specific affinity column
2) Protein A or G column |
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Protein A column (Rabbit IgG) or
Protein G column (Mouse IgG) |
| Serum amount |
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10 ㎖ |
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10 ㎖ |
| Period |
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2-3 weeks |
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2-3 weeks |
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Client Requirements
- Serum or ascites amounts of 10 ㎖ that are being purified.
- Antigen (Protein 2.5 ㎎, Peptide 5 ㎎, only when chosen for antigen
specific antibody) |
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Consulting/ordering methods
For mode details regarding our service and
large-scale production,
please call +82-2-6933-6783, or e-mail us at :
orders@younginfrontier.com
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Related Resource
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